The Anti-Inflammatory Potential of a Combination of Centella asiatica Leaf and Caesalpinia sappan Wood Ethanol Extracts

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Authors

  • IRMA SANTI - UNIVERSITAS MUSLIM INDONESIA
  • Sukmawati Sukmawati
  • Etri Wijayanti

DOI:

https://doi.org/10.24252/djps.v8i2.56478

Keywords:

Anti-inflammatory, ethanol extract, , Centella asiatica L., Caesalpinia sappan L., carrageenan

Abstract

Introduction: Inflammation is the body's response to infection and tissue damage by sending the body's cells and defense molecules from the bloodstream to the location needed to eliminate the cause of the damage. Centella asiatica L. and Caesalpinia sappan L. contain tannin, phenolic, and flavonoid compounds that are effective as anti-inflammatories.  Objective: This study aimed to test the anti-inflammatory potency and effective dose  of Centella asiatica leaves and Caesalpinia sappan  wood, in male rats (Rattus norvegicus). Methods: 20 wistar rats were divided into five treatment groups: a negative control group (CMC Na 1%), a positive control group (Diclofenac Sodium), and three combination groups of a combination  Centella asiatica leaves and Caesalpinia sappan wood ethanol extracts (EEPS) with dose variations of 250:375 mg/kgBW, 200:400 mg/kgBW, and 150:425 mg/kgBW. Intraplantar edema induction with lambda carrageenan. parameter measured of edema volume with a plethysmometer and edema thickness with a caliper. Results: The combination of ethanol extract showed anti-inflammatory effects at all doses, which showed a decrease in the volume and thickness of edema at 180 to 360 minutes. At the dose 150:425 mg/kgBW shows total inflammatory response during the observation period is seen from the area under curve (AUC)  value where AUC value of edema volume of 21.50 and an AUC value of edema thickness of 46.70. In addition, at this dose, percentage of anti-inflammatory activity (%DAI) where the value of %DAI at edema volume is 34% and %DAI at edema thickness is 22%. Conclusion: The combination of ethanol extract has an anti-inflammatory effect, as well as an effective dose to reduce the volume and thickness of edema in male rats (Rattus norvegicus), namely a dose of 150:425 mg/kgBW.

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References

Acebo, I. G.-, Sotos, T. D., De Pedro, M., Pérez-Gómez, B., Castaño-Vinyals, G., Fernández-Villa, T., Palazuelos-Calderón, C., Amiano, P., Etxeberria, J., Benavente, Y., Fernández-Tardón, G., Salcedo-Bellido, I., Capelo, R., Peiró, R., Marcos-Gragera, R., Huerta, J. M., Tardón, A., Barricarte, A., Altzibar, J. M., … Llorca, J. (2018). Epidemiology of non-steroidal anti-inflammatory drugs consumption in Spain. The MCC-Spain study. BMC Public Health, 18(1134), 1–13. https://doi.org/10.1186/s12889-018-6019-z

Asevedo, E. A., Ramos Santiago, L., Kim, H. J., Syahputra, R. A., Park, M. N., Ribeiro, R. I. M. A., & Kim, B. (2024). Unlocking The Therapeutic Mechanism of Caesalpinia Sappan: A Comprehensive Review of Its Antioxidant and Anti-Cancer Properties, Ethnopharmacology, and Phytochemistry. In Frontiers in Pharmacology (Vol. 15). Frontiers Media SA. https://doi.org/10.3389/fphar.2024.1514573

Apridamayanti, P., Sanera, F., & Robiyanto, R. (2018). Aktivitas Antiinflamasi Ekstrak Etanol Daun Karas (Aquilaria malaccensis Lamk.). Pharmaceutical Sciences and Research, 5. (3), 152–158. https://scholarhub.ui.ac.id/psr/vol5/iss3/6/

Azizah, M., & Azhairi. (2017). Uji Efek Antiinflamasi Kombinasi Ekstrak Daun Pecut Kuda (Stachytarpheta Jamaicencis L.) Dan Madu Hutan Pada Tikus Putih Jantan Galur Wistar Yang Diinduksi Albumin Telur 5 %. Jurnal Penelitian Farmasi Indonesia, 6(1), 7–14. https://doi.org/https://ejournal.stifibp.ac.id/index.php/jibf/article/view/25

Diniz, L. R. L., Calado, L. L., Duarte, A. B. S., & de Sousa, D. P. (2023). Centella asiatica and Its Metabolite Asiatic Acid: Wound Healing Effects and Therapeutic Potential. In Metabolites (Vol. 13, Issue 2). MDPI. https://doi.org/10.3390/metabo1302 0276.

Fadlilah, A. R., & Lestari, K. (2023). Review: Peran Antioksidan dalam Imunitas Tubuh. Jurnal Farmaka. 21 (2) Jurnal.unpad.ac.id/farmaka/article/view/45929

Hasty Martha Wijaya, R. N. L. (2021). Formulasi dan Evaluasi Fisik Sediaan Suspensi Kombinasi Ekstrak Biji Pepaya (Carica Papaya L.) Dan Umbi Rumput Teki (Cyperus Rotundus L.) Dengan Variasi Konsentrasi Suspending Agent PGA (Pulvis Gummi Arabici) dan CMC-Na (Carboxymethylcellusum Natrium) Cendekia journal of Pharmacy, 5(2), 66–175.

Ikrima, K., Amalia, R., & Levita, J. (2020). Peran Spesies Oksigen Reaktif pada Inflamasi Serta Antioksidan Alami Sebagai Fitoterapi . Jurnal Farmaka 17 (3).https://jurnal.unpad.ac.id/farmaka/article/view/22010.

Kresnamurti, A., Hardiyono, Siswulandari, F., & Hamid, I. S. (2021). Aktivitas Antiinflamasi Ekstrak Etanol Echinometra mathaei pada Tikus Putih Jantan dengan Induksi Karaginan. Pharmacon: Jurnal Farmasi Indonesia, 18(2), 141–147. https://doi.org/10.23917/pharmacon.v0i0.15021

Kumar, V., Frcpath, M. M., Abbas, A. K., & Aster, J. C. (2020). Buku Ajar Patologi Robbins-E-Book. Singapore: Elsevier.

Legasari, L., Wati, A., & Yani, D. F. (2023). A Comparison of Anti-inflammatory Activity of Ethanol Extracts of Young and Old Areca Seeds (Areca catechu L.) in vitro. Stannum : Jurnal Sains dan Terapan Kimia, 5(2), 93–99. https://doi.org/10.33019/jstk.v5i2.4376

Maulana, I., Roddu, A. K., & Suriani, S. (2020). Uji Efektifitas Ekstrak Kulit Petai (Parkia speciosa Hassk) Terhadap Mencit (Mus musculus) Sebagai Anti Inflamasi. Lumbung Farmasi: Jurnal Ilmu Kefarmasian, 1(2), 80–84. https://doi.org/10.31764/lf.v1i2.2559

Mueller, M., Weinmann, D., Toegel, S., Holzer, W., Unger, F. M., & Viernstein, H. (2016). Compounds from Caesalpinia sappan with Anti-inflammatory Properties in Macrophages and Chondrocytes. Food and Function, 7(3), 1671–1679. https://doi.org/10.1039/c5fo01256b

Ma Chao., & Zhang, J.-Ming. (2015). Rat Paw Oedema Modeling and NSAIDs: Timing of Effects (27th ed.). International Journal of Risk & Safety in Medicine. https://doi.org/103233/JRS150697

Puspitadewi, N. Sriwidodo S (2023). Review Artikel: Aktivitas dan Pemanfaatan Brazilin Dari Kayu Secang (Caesalpinia Sappan L.) dalam Sediaan Kosmetik. Jurnal Farmaka 21 (1). https://jurnal.unpad.ac.id/farmaka/article/view/37702

Putri, B. M., Sugiarto, Wasita, B., & Febrinasari, R. P. (2021). Antioxidant Activity of Ethanol Extract of Secang Wood (Caesalpinia sappan L.) , Gotu Kola (Centella asiatica L . ), and Their Combinations with DPPH Assay. 2nd International Conference of Health, Science and Technology, 45–49. https://doi.org/10.47701/icohetech.v1i1.1081

Putri, B. M., Wasita, B., & Febrinasari, R. P. (2022). The Effect of Combined Extracts of Sappan Wood (Caesalpinia sappan L.) and Gotu Kola (Centella asiatica L.) in Improving Diabetic Condition in Rats. Jurnal Gizi dan Pangan, 17(1), 37–46. https://doi.org/10.25182/jgp.2022.17.1.37-46

Pramitaningastuti, A. S., & Anggraeny, E. N. (2017). 'Uji Efektivitas Antiinflamasi Ekstrak Etanol Daun Srikaya (Annona Squamosa. L) terhadap Udema Kaki Tikus Putih Jantan Galur Wistar. Jurnal Ilmiah Farmasi. 13 (1) , 8-13. https :/journal.uii.ac.id/JIF/article/view/8803

Sadik, F., & Anwar, R. A. (2022). Standarisasi Parameter Spesifik Ekstrak Etanol Daun Pegagan (Centella asiatica L.) Sebagai Antidiabetes. Journal Syifa Sciences and Clinical Research, 4(1), 1–9. ttps://doi.org/10.37311/jsscr.v4i1.13310

Salsabila, A. F., & Fuadi, A. M. (2023). Pengaruh Waktu Maserasi dan Konsentrasi Pelarut Etanol Terhadap Rendemen dan Aktivitas Antioksidan Kayu Secang. Jurnal Teknik Indonesia, 2(2), 87–100. https://doi.org/10.58860/jti.v2i2.16

Setiawan, B., & Ernawati. (2024). Peran Nutrasetikal dalam Mitigasi Inflamasi Kronis : Tinjauan Pustaka. Calvaria Medical Journal, 2(1), 29–35. https://doi.org/10.30742/cmj.v2i1.44

Sriarumtias, F. F., Ardian, M. E., & Najihudin, A. (2020). Uji Aktivitas Ekstrak Daun Jeruk Manis ( Citrus x aurantium L .) sebagai Antiinflamasi Anti-Inflammatory Activity of Sweet Orange ( Citrus x aurantium L .) Leaves Extract. Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia), 17(01), 197–206. https://doi.org/10.30595/pharmacy.v17i1.6541

Suryandari, S. S., Queljoe, E. de, & Datu, O. S. (2021). Uji Aktivitas Antiinflamasi Ekstrak Etanol Daun Sesewanua (Clerodendrum squamatum Vahl.) Terhadap Tikus Putih (Rattus norvegicus L.) yang Diinduksi Karagenan. Pharmacon, 10(3), 1025–1032. https://doi.org/10.35799/pha.10.2021.35606

Salsabila, F., Posangi, J., Mambo, C. D., Fatimawali, F., Regina Masengi, A. S., & Nangoy, E. (2025). Profil Fitokimia dan Uji Aktivitas Antioksidan Ekstrak Etanol Daun Pacar Air Menggunakan Metode Abts (2,2’-Azinobis(3-Ethylbenzothiazoline-6-Sulfonic Acid). Jurnal Locus Penelitian Dan Pengabdian, 4(2), 1154–1169. https://doi.org/10.58344/locus.v4i2.3805

Thirza, S. Q., Pratiwi, M. D., Evan, D., Putra, P ., Sriepinndonta, P. M., Fitriani, F. N., Kalsum, U., Mintaroem, K., & Norahmawati, E. (2021). Anti-Inflammatory Effects of Centella asiatica Ethanolic Extracts towards Indomethacin-Induced Gastric Ulcer Model in Rats by Altering COX-2 Expression. AIP Conference Proceedings, 2353(1). https://doi.org/10.1063/5.0053025

Widowati, W. P. I. K. (2011). Uji Fitokimia dan Potensi Antioksidan Ekstrak Etanol Kayu Secang ( Caesalpinia sappan L .). Maranatha Journal of Medicine and Health, 11(01), 23–31. https://doi.org/http://majour.maranatha.edu/index.php/jurnal-kedokteran/article/view/900

Wirawati, K. T., Ompusunggu, G. B., Wardani, L., & Yanti, K. R. D. (2025). The Pharmacological Potential of Sappan Wood (Caesalpinia sappan L.): A Review of Recent Evidence. Pharmacy Reports, 3(3), 86. https://doi.org/10.51511/pr.86

Zuo, L., Prather, E. R., Stetskiv, M., Garrison, D. E., Meade, J. R., Peace, T. I., & Zhou, T. (2019). Inflammaging and Oxidative stress in Human Diseases: from Molecular Mechanisms to Novel Treatments. In International Journal of Molecular Sciences (Vol. 20, Issue 18). MDPI AG. https://doi.org/10.3390/ijms20184472

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Published

2025-12-30

How to Cite

-, I. S., Sukmawati, S., & Wijayanti, E. (2025). The Anti-Inflammatory Potential of a Combination of Centella asiatica Leaf and Caesalpinia sappan Wood Ethanol Extracts: -. Ad-Dawaa’ Journal of Pharmaceutical Sciences, 8(2), 209–219. https://doi.org/10.24252/djps.v8i2.56478

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Vol. 8 No.2 (2025) (on progress)

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